Paper 10: Characterization of Bone in Osteochondral Allografts Utilizing Inflammatory-Mediated Cellular Death Pathways

Objectives: Osteochondral allografts are used to treat chondral and osteochondral defects. While prior studies have correlated cell death within the component of directly with clinical outcomes, subchondral trabecular bone has not been well characterized. demonstrated a role for apoptosis in allografts, none examined pyroptosis necroptosis allografts. These pathways particular relevance because persistent inflammation associated on imaging is poor patient-reported outcomes. We report here study examining mechanism Methods: Fresh human talus were cultured 14, 28, or 42 days at 4 o C standard media. plugs taken specified time points fixed 10% formalin. Cell was measured cartilage, bone, TUNEL staining. The further staining (activated caspase 3), (MLKL), (cleaved gasdermin D). Percentage cells positive calculated each zone (cartilage, bone). Each point included 4-5 replicates. Appropriate statistical comparisons made between groups. Results: significantly greater than cartilage. There an increase over cartilage as bone. Apoptosis most prevalent death, but necrosis present all areas Conclusions: This suggests that allograft outcomes may be related viability bony allograft. versus percentage through pyroptosis, inflammatory-mediated pathway. Culture media surgical strategies limit effect inflammatory help improve postoperative incorporation control can complicate